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. 2016 Jan 21;47:20. doi: 10.1186/s13567-016-0306-4

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© Holzer et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PPRV viraemia after challenge. Blood was collected from all animals at 2, 4, 6, 8, 10 and 12 dpi and stored at −70 °C until assay. Real-time PCR was used to measure PPRV RNA, which is plotted as 45-Ct, where Ct is the threshold cycle. A data from negative control animals; B data from group 5 animals. All other samples were completely negative for PPRV RNA.