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Expression levels of hsp60, hsp70 and hsp90 by C. caretta embryos from a temperate (DHI, hatched bars; n = 129–130 individuals per gene) and sub-tropical (BB, grey bars; n = 30 individuals per gene) rookery in response to an acute heat shock. All mRNA data are normalized to the reference gene 18s (values are clutch mean ± s.e.m.; N = 18). Significant differences (p < 0.05) between rookeries denoted with an asterisk.
