Fig. 1.

Associated spontaneous production of both IL-17A and IL-4 and expression of CD161, CCR8 and CCR4 by fresh decidua CD3+CD4+ T cells in successful pregnancy. a Unstimulated decidual and PB CD4+ T cells purified from the same 9 pregnant women were cultured for 24 h and IL-4, IL-17A, IL-17F and IL-22 production (mean ± SEM) was measured. b RT-PCR of unstimulated decidual and PB CD4+ T cells purified from the same 3 pregnant women for IL-17A, IL-4 and RORC was performed. c, d Th1 cells express CXCR3, whereas Th2 cells express CCR4, CCR8 and CRTH2 and Th17 cells express CD161, IL-23 receptor (IL-23R) CCR6 and CCR4. The associated expression of molecules expressed by Th17 cells and by Th1 and Th2 cells in fresh unstimulated CD4+ T cells purified from decidua and PB of the same pregnant women were analyzed by 4-color (CD3, CD4, CD161 and CCR4 or CCR8 or CXCR3) flow cytometry. c is a representative experiment and d data are represented as mean ± SEM. For a, b and d the statistical analysis was performed with Wilcoxon test