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. Author manuscript; available in PMC: 2016 Dec 4.
Published in final edited form as: J Mol Biol. 2015 Oct 30;427(24):3890–3907. doi: 10.1016/j.jmb.2015.10.015

Table 1.

SAXS Parameters for Full-length CheA and Select Variants Bound to Nucleotides

Protein Nucleotide Rg (Å) dmax (Å) ravg (Å) Px
CheAFL none 72.7 170 74.1 2.3
ADP 73.1 170 76.4 2.4
ATP 73.8 170 74.3 2.2
ADPCP 57.7 170 81.0 0.9
P3P4P5 none 54.4 130 61.9 2.8
ADP 50.2 130 59.3 2.7
ADPCP 50.2 130 59.8 2.7
CheAFL D449A none 60.3 170 77.2 2.5
ADPCP 64.0 170 76.5 2.4

Rg represents the radius of gyration determined by Guinier analysis

dmax represents the maximum distance constraint in the pairwise distance distribution or P(r) generation.

ravg is the average radius from the P(r) distribution

Px is the Porod exponent; larger values represent more compact particles.

Above parameters were determined with Scatter: (biosis.net)