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. Author manuscript; available in PMC: 2016 Dec 4.
Published in final edited form as: J Mol Biol. 2015 Oct 30;427(24):3890–3907. doi: 10.1016/j.jmb.2015.10.015

Table 3.

Kinetic parameters for CheA autophosphorylation.

Components A0 (CheA-P
formation)		 k1 (min−1) k2 (min−1) k1A0 R2
P1+P3P4P5 25 °C 1.3 ± 0.08 0.009 ± 0.007 - 0.011 0.98
P1+P3P4P5 37 °C 0.54 ± 0.04 0.040 ± 0.006 - 0.022 0.99
P1+P3P4P5 42 °C 0.33 ± 0.08 0.13 ± 0.10 - 0.043 0.60
P1+P3P4P5 55 °C 0.73 ± 0.01 0.31 ± 0.02 - 0.23 0.99
CheAFL 25 °C 1.14 ± 0.09 0.06 ± 0.01 - 0.068 0.97
CheAFL 37 °C 0.358 ± 0.001 0.216 ± 0.004 - 0.0773 0.99
CheAFL 42 °C 0.5 ± 0.1 1.3 ± 0.9 0.01 ± 0.009 0.65 0.82
CheAFL 55 °C 1.0 ± 0.3 1.2 ± 0.7 0.03 ± 0.02 1.2 0.86
P1+P3P4 55 °C 0.96 ± 0.05 0.11 ± 0.02 - 0.11 0.97
CheAFL+P3P4P5 55 °C 0.52 ± 0.05 0.15 ± 0.04 - 0.078 0.97
Mixed CheAFL+P3P4P5 55 °C 0.70 ± 0.03 0.24 ± 0.04 - 0.17 0.97
CheAFL+ADP 55 °C 0.510 ± 0.003 0.66 ± 0.02 - 0.335 0.99
P1+P3P4P5+ADP 55 °C 0.52 ± 0.05 0.24 ± 0.06 - 0.12 0.96

Data were fit to the first order rate equation (Pt = A0(1−e−k1t)), except for the biphasic behavior of CheAFL at 42 °C and 55 °C; in these case k2 represents the rate constant for P1-P decay to P1#