KSHV stimulates YAP/TAZ activity. (a) IHC staining of YAP/TAZ, YAP, TAZ, or LANA in KSHV-negative or KSHV-positive tumors generated from mECK36 cells. (b) Expression of lytic genes (vGPCR, RTA, K8.1) in adherent HEK293 cells (HEK293A) transfected with recombinant KSHV. Expression of lytic genes was induced by treating cells with 3 mM sodium butyrate for 48 hrs. (c) Expression of lytic KSHV genes induces YAP/TAZ activity. Protein lysates from serum starved adherent HEK293 cells infected with or without KSHV were prepared and subjected to immunoblotting.