Table 1.

Primer sequences and PCR conditions

Target	Primer	Sequences (5′-3′)	PCR conditions
Total HIV-1 DNA	M667/AA55M	GGC TAA CTA GGG AAC CCA CTG / GCT AGA GAT TTT CCA CAC TGA CTA A	95°C 3 min - 95°C 30s - 60°C 30s- 72°C 30s - 72°C 5 min for 35 cycles
	M667/BB301	GGC TAA CTA GGG AAC CCA CTG/ CCC TGT TCG GGC GCC ACT G	95°C 3 min - 95°C 30s - 60°C 30s- 72°C 30s - 72°C 5 min for 35 cycles
	M667/M661	GGC TAA CTA GGG AAC CCA CTG / CCT GCG TCG AGA GAT CTC CTC TGG	95°C 3 min - 95°C 30s - 60°C 30s- 72°C 30s - 72°C 5 min for 35 cycles
Integrated HIV-1 DNA
1st PCR	Nef Alu1 Alu2	ATG CTG CTT GTG CCT GGC TAG AAG C TCC CAG CTA CTG GGG GAG GCT GAG G GCC TCC CAA AGT GCT GGG ATT ACA G	95°C 3 min - 95°C 30s - 60°C 30s- 72°C 3 min - 72°C 5 min for 18 cycles
Nested PCR	AA55M M667	GCT AGA GAT TTT CCA CAC TGA CTA A GGC TAA CTA GGG AAC CCA CTG	95°C 5 min - 95°C 30s - 60°C 30s- 72°C 30s - 72°C 3 min for 25 cycles
2-LTR circles	9600 515c	GCT TAA GCC TCA ATA AAG CTT GCC T GTG TGT AGT TCT GCT AAT CAG GGA A	95°C 5 min - 95°C 30s - 60°C 30s- 72°C 30s - 72°C 10 min for 40 cycles
β-Actin	Actin F Actin R	ATC TGG CAC CAC AAC AAT GAG CTG CG CGT CAT ACT CCT GGA TCC ACA TCT GC	94°C 2 min - 94°C 30s - 55°C 30s- 72°C 1 min - 72°C 10 min for 25 cycles