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. Author manuscript; available in PMC: 2016 Jan 21.
Published in final edited form as: Biochemistry. 2007 Feb 27;46(12):3862–3871. doi: 10.1021/bi061999r

Figure 2.

Figure 2

Steady-state tryptophan fluorescence emission of the PerCter in the presence CaM. Representative spectra of 10 μM PerCter (—) and 10 μM PerCter upon the addition of 20 μM CaM (---). Spectra were recorded in 10 mM HEPES at pH 7.3 and 100 mM KCl with (A) 10 mM CaCl2 or (B) 4 mM EGTA. (C) Representative spectra of 10 μM Scr. Helix (−) and 10 μM Scr. Helix upon the addition of 20 μM CaM (---). Spectra were corrected for background and dilution before being smoothed over a 5-point average. All samples were excited at 295 nm. These spectra represent at least six series of experiments.