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. 2015 Jun 22;35(3):e00210. doi: 10.1042/BSR20150042

Figure 3. PP5–Hsp90 complex formation is unaffected by the P5SAs.

Figure 3

(A) Complex formation between PPH-5 and CeHsp90/DAF-21 is not influenced by the presence of the P5SAs. The sedimentation coefficient s20,w of YFP–Hsp90 is shifted from 6.8 S (black) to 8.9 S in presence of PPH-5 (red). Supplementation of different P5SAs (orange) does not alter the sedimentation coefficient of the phosphatase–chaperone complex. Presence of the Hsp90-derived MEEVD peptide shifts the s20,w value from 8.9 to 7.6 S (green). P5SAs were added at their maximal soluble concentrations (B) Titration of PPH-5 phosphatase activity with P5SA-5 in absence of MEEVD peptide is depicted in black whereas activities in presence of 60 μM peptide are shown in red. Results are expressed as mean ± S.D. (n≥3).