Figure 4. Melting of the Cyp40 TPR domain monitored by tryptophan fluorescence.

(A) The three tryptophan residues of hCyp40 are located in the TPR domain at key interfaces between the three tandem TPRs. Helices are labelled 1–6. (B) Steady state-fluorescence is strongly influenced by both temperature and ligand binding. (C) Average emission wavelength increases with temperature and is decreased upon ligand binding.