Table 1. Theoretical and experimental inter-spin distances.
Experimental mean inter spin distance (nm) | ||||||
---|---|---|---|---|---|---|
Mutant | apo | GppNHp | GDP•AlFx | GDP | Cβ–Cβ distance | Calculated (MD-RLA) mean distance (nm) |
S928R1COR | 2.61 | 2.68 | 2.51 | 2.59 | 2.5 | 2.82 |
(2.24)* | (2.28)* | (2.23)* | (2.19)* | |||
T476R1Roc | 3.19 | 3.35 | 3.21 | 3.39 | 2.3 | 2.84 |
+ L487A | 2.75 | 3.24 | 2.40 | 2.74 | ||
+ Y558A | 3.26 | 3.01 | 3.07 | 3.10 | ||
S542R1Roc | 3.00 | 3.05 | 2.87 | 3.12 | 2.1 | 2.63 |
+ L487A | 2.81 | 2.92 | 3.14 | 2.88 | ||
+ Y558A | 2.77 | 2.76 | 2.65 | 2.66 | ||
C600R1Roc | 3.74 | 3.73 | 3.46 | 3.37 | 3.5 | 4.42 |
+ L487A | 3.15 | 3.12 | 2.79 | 3.35 | ||
+ Y558A | 2.95 | 2.74 | 2.41 | 2.64 |
*Maximum of the major peak in the experimental distance distribution.