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. Author manuscript; available in PMC: 2016 Jan 21.
Published in final edited form as: J Clin Exp Ophthalmol. 2015 Oct 27;6(5):491. doi: 10.4172/2155-9570.1000491

Figure 5.

Figure 5

PEDF-R is constitutively expressed by RGCs and Müller cells in healthy retina. A. Graphical representation of pedf-r total mRNA levels in naïve C57 retina as measured by qRT-PCR. Y-axis represents the average delta of threshold cycle (Δ-CT) values for pedf-r normalized to the control gene gapdh. B. Representative micrograph of longitudinal sections of retina from naïve mice immunolabeled with antibody against PEDF-R. Morphology consistent with localization to RGCs (white arrowheads) and Müller cells (black arrowheads). C. Retinal layer-specific quantification of PEDF-R labeling, expressed in intensity (arbitrary units) per area (mm2). All asterisks denote p<0.05. D. Representative confocal micrographs with orthogonal view (bottom panel) of wholemount retina from naïve mice co-immunolabeled with antibodies against PEDF-R (green) and the RGC marker SMI-31 (red). PEDF-R localizes to SMI-31+RGC soma and axons, as indicated by the yellow appearance of punctate immunolabeling (arrowheads). E. Representative confocal micrographs with orthogonal view (bottom panel) of wholemount retina from naïve mice co-immunolabeled with antibodies against PEDF-R (green) and the Müller cell marker glutamine synthetase (GluSyn; blue). PEDF-R co-localizes with Müller cell endfeet in the NFL, as indicated by the white appearance of immunolabeling (arrowheads). ONL: Outer Nuclear Layer; OPL: Outer Plexiform Layer; INL: Inner Nuclear Layer; IPL: Inner Plexiform Layer; GCL: Ganglion Cell Layer; NFL: Nerve Fiber Layer.