Figure 6.

Elevated IOP increases PEDF-R expression in murine retina. A. Graphical representation of pedf-r total mRNA in retina from saline- and microbead-injected mice, as measured by qPCR. y-axis represents fold change in Δ-CT values for pedf-r (normalized to the control gene gapdh) for microbead-injected versus saline-injected. Asterisk denotes p<0.05. B. Representative micrographs of immunolabeling for PEDF-R in longitudinal sections of retina from saline- (right panel) and microbead-injected (left panel) mice demonstrate increased intensity of labeling, particularly in the IPL, GCL and NFL, as compared to saline-injected eyes. The pattern of PEDF-R immunolabeling is consistent with RGC soma in the GCL (white arrowheads) and Müller cell processes and endfeet in the ONL and NFL (black arrowheads), respectively. C. Retinal layer-specific quantification of PEDF-R labeling, expressed in intensity (arbitrary units) per area (mm²). All asterisks denote p<0.05. ONL: Outer Nuclear Layer; OPL: Outer Plexiform Layer; INL: Inner Nuclear Layer; IPL: Inner Plexiform Layer; GCL: Ganglion Cell Layer; NFL: Nerve Fiber Layer