Table 1.
Primary antibodies used for immunohistochemistry and immunocytochemistry. Primary antibodies, sources, and concentrations used for detection of PEDF, PEDF-R and labeling of RGCs and Müller cells in murine retina.
| Target | Antibody name | Concentration used |
Vendor | Catalog # |
|---|---|---|---|---|
| PEDF | Mouse anti-human PEDF | 20 µg/ml | Abcam (Cambridge, MA) | 115489 |
| PEDF-R | Goat anti-human PEDF-R/PNPLA2 | 20 µg/ml | R&D Systems (Minneapolis, MN) | AF5387 |
| Goat anti-human Adipose Triglyceride Lipase | 2.5 µg/ml | Abcam (Cambridge, MA) | 85858 | |
| Retinal ganglion cell (RGC) | Mouse anti-mammalian SMI-31 (phosphorylated neurofilaments) | 1 µg/ml | Covance (Princeton, NJ) | SMI-31R |
| Rabbit anti-mammalian Gamma Synuclein | 10 µg/ml | Abcam (Cambridge, MA) | 55424 | |
| Müller Cells | Goat anti-human Glutamine Synthetase | 4 µg/ml | Santa Cruz Biotechnologies (Santa Cruz, CA) | Sc-6640 |
| Rabbit anti-human Glutamine Synthetase | 4 µg/ml | Santa Cruz Biotechnologies (Santa Cruz, CA) | Sc-6640-R |