Figure 6.

Protein expression of p53 and p53-dependent genes and detection of apoptosis in the colorectum of Ugt1^F/Fand Ugt1^ΔIECmice with colorectal neoplasia after AOM and DSS treatment. Colorectum tissues from AOM plus DSS-treated Ugt1^ΔIEC and Ugt1^F/F mice were collected and analyzed. (A) Expression levels of UGT1A, p53, p21, Bax, and cleaved caspase-3 were examined by Western blot analysis with GAPDH as a loading control. Quantitation of the Western blots is shown in Supplementary Figure 5. (B) TUNEL staining of colon sections (100× magnification). (C) Quantification of the number of TUNEL positive cells.