Supplementary Figure 2.

P53 gene expression analysis. Human HT29 and LS180 cells treated with actinomycin D or etoposide after siRNA knockdown of the UGT1 locus were used to quantitate by real time PCR gene expression of the p53 gene. Similar quantitation was performed using intestinal RNA from Ugt1F/F and Ugt1ΔIEC mice after DSS treatment to induce intestinal inflammation.