Supplementary Figure 4.
No marked changes in the severity of ulcer or inflammatory gene expression in colon tissues between Ugt1ΔIECand Ugt1F/Fmice after DSS treatment. Colon tissues from 3% DSS-treated or untreated Ugt1ΔIEC and Ugt1F/F mice were used to prepare total samples of total RNA. (A) Expression of inflammatory genes was quantitated by real-time PCR, and normalized to the level of cyclophilin mRNA. (B) Colitis score quantified by stool occult blood, rectal prolapse, and diarrhea. Results are presented as mean ± standard deviation of at least four mice. (C) Quantification of the number of ulcers by analyzing H&E-stained sections. (D) Colon ulcers are shown by H&E staining (200× magnification).