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. 2016 Jan 21;11(1):e0146622. doi: 10.1371/journal.pone.0146622

Fig 6. Percentage of IFN-γ- and IL-10-producing CD3+ lymphocytes after co-culture with either unloaded or whole tumour-lysate pulsed DCs.

Fig 6

Syngeneic naïve T-cells from MMTV-Ras mice were cultured with either unloaded DCs or TAA-DCs for 1-3-5-7- days. After the indicated days of DC-T culture, BFA was added to the culture for the last 6 hours before staining to prevent cytokine secretion. Cell were then stained with anti-CD3-PECy7 and anti-CD11c PECy5 mAbs, fixed, permeabilized, stained with PE- or FITC-conjugated mAbs for intracellular cytokines, and subjected to flow cytometry. Percentage of IFN-γ-expressing CD3+ lymphocytes (A), IL-10-expressing CD3+ lymphocytes (B), IL-12p70-expressing CD11c+ cells (C) and IL-10-expressing CD11c+ cells (D). Data represents three different lots of independent experiments. Mean values ± SD and statistically significant differences are indicated. For each analysis, 20,000 events were acquired and gated on either CD3 or CD11c expression and side scatter properties. *P value < 0.05; **P value <0.005.