Fig. 6.

a HDAC2, CAPZA1, and TXNL1 immunohistochemical detection in colorectal cancer specimens based on a tissue microarray. Image examples are given at a 800-fold magnification (800×). Immunopositivity for individual protein markers was assessed semiquantitatively as described in “Materials and methods”. b Tissue-microarray-based immunohistochemical evaluation of HDAC2, CAPZA1, and TXNL1 comparing diploid versus aneuploid colorectal carcinoma specimens. Immunoreactivity was scored with “0” showing no positivity, “1” presenting up to 20% immunopositive cells, “2” up to 50%, and “3” above 50% stained cells. Barplots of the TMA analysis confirmed HDAC2 and TXNL1 as significantly (asterisk) differentially expressed proteins between diploid and aneuploid tumors