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. 2016 Jan 21;11(1):e0147161. doi: 10.1371/journal.pone.0147161

Fig 1. Aspirin inhibited preadipocyte differentiation and fat storage in mature 3T3-L1 adipocytes.

Fig 1

A. Cell proliferation of 3T3-L1 cells treated with increasing doses of aspirin for 48 h, assessed by MTT assay. B. Cell morphology of oil accumulation in 3T3-L1 adipocytes treated with aspirin during preadipocyte differentiation; mature cells were stained with Oil Red O and observed microscopically at 200× magnification. C. The oil accumulated in 3T3-L1 cells was dissolved in 2-propanol and quantified by reading the absorbance at 500 nm using a microplate reader. Data are presented as mean ± SEM. Statistical analysis was done by one-way ANOVA and LSD post hoc test; significantly different at * p < 0.05 or # p < 0.001 vs. control group.