Table 5.
Phenotypes of C. albicans mutants.
C. albicans strain | Generation time 30 °C (min) | Stress | Morphology | Nutrition | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cell Wall | Osmotic | Oxidative | T° | |||||||||||
Sensitivity to Calcofluor White 28 μg/ml | Calcofluor White 28 μg/ml + 1 M sorbitol | Sensitivity to Congo Red 175 μg/ml | Congo Red 175 μg/ml + 1 M sorbitol | Sensitivity to Sorbitol 1.5 M | Sensitivity to KCl 1.2 M | Sensitivity to Menadione 0.2 mM | Sensitivity to H2O2 100 mM | 45 °C | Serum 10% 37 °C | Spider 30 °C | Spider 37 °C | C-Source: Mannitol | ||
SC5314 | 72 | Control Strain (wild-type) | ||||||||||||
SN250 | n.d. | Collection Control Strain | ||||||||||||
ali1 | 100 | + | √ | + | √ | + | + | + | + | + | + | + | + | + |
mci4 | 88 | + | + | + | + | + | + | + | ||||||
orf19.287 | 96 | + | √ | + | √ | + | + | + | + | + | + | + | + | |
orf19.3060 | n.d. | + | √ | + | √ | + | ||||||||
orf19.3290 | n.d. | + | + | + | ||||||||||
orf19.5352 | 71 | + | √ | + | √ | |||||||||
orf19.7590 | 99 | + | √ | + | + | + | + | + | + | + | ||||
pst3 | n.d. | + | √ | + | √ | + | + | |||||||
tos1 | 73 | + | √ |
Sensitivity to the compounds was indicated with “+” and means reduction of growth relative to the controls SC5314 and SN250. Blank box stand for the same growth as the controls. “n.d.” or Not Determined. Stress media growth, colony morphology and nutrient media growth phenotypes were indicated in the table. The data shown are from at least three replicates. 8 out 17 mutants studied (ihd1, orf19.3335, orf19.6553, orf19.7196, orf19.7238, pga45, ptp3 and ycp4) not exhibit any phenotype.