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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: Dev Biol. 2015 Jul 21;415(2):383–390. doi: 10.1016/j.ydbio.2015.07.018

Fig. 2.

Fig. 2

Increase of ADAM13 expression leads to higher CNC invasiveness in vitro. Confrontation assay. Left column: Confronted explants at time point t=0. Right column: Confronted CNC explants at time point of highest invasion Δt. Confrontations of wildtype explants with (A) wildtype CNC explants, (B) explants of ADAM13-depleted CNC (MO13) or (C) CNC explants overexpressing C11 show only small overlapping explant borders with low invasiveness. In contrast, yellow overlapping area increases significantly in confrontations with CNC cells overexpressing (D) ADAM13 or (E) cleavage product EC1-3. (F) Overexpression of the non-adhesive extracellular fragment na-EC1-3 in CNC explants leads to small overlap when confronted with wild-type explants. Average Overlapping Index (OI) is given in (G) with n=number of confrontations. wt: wildtype. Error bar shows standard error. (***) Significance to wildtype vs. wildtype with p < 0.005 after Student's t-test. Scale bar, 50 μm.