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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: Dev Biol. 2015 Jul 21;415(2):383–390. doi: 10.1016/j.ydbio.2015.07.018

Fig. 3.

Fig. 3

Increase of ADAM13 expression blocks the repulsive response in colliding single CNC cells in vitro. Collision assay. First three columns: Single CNC cells before (t-Δt), during (t) and after (t+Δt) mutual contact. Yellow and white vectors give change of cell location. Fourth column: Relative velocity vectors with initial velocity vector (red, n=10 collisions). Mutual collisions of (A) wildtype CNC cells, (B) ADAM13-depleted CNC cells (MO13) and (C) CNC cells overexpressing C11 lead to repulsive response of the cells and CIL. In contrast, CNC cells overexpressing (D) ADAM13 or (E) the cleavage product EC1-3 show no change of direction. (F) Repulsive response and CIL are observed in collisions in single CNC cells over-expressing the non-adhesive fragment na-EC1-3. Scale bar, 50 μm. (G) Average velocity of single cells in the various conditions. Overexpression of cadherin-11 and expression of EC1-3 or na-EC1-3 significantly reduce the cell velocity.