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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: Dev Biol. 2015 Jul 21;415(2):383–390. doi: 10.1016/j.ydbio.2015.07.018

Fig. 5.

Fig. 5

EC1-3 does not promote migration in vivo through the homophilic site. (A–D) Targeted injection assays to measure CNC migration with histograms showing the percentage of embryos with inhibited migration normalized to RFP (A, C) and representative images of each condition with arrowheads pointing to RFP-positive cells within the branchial and hyoid arches (B, D). na-EC1-3 rescues the overexpression of C11 or C11-egf as well as EC1-3 (A, B), and also rescues migration when expressed along with MO11 and C11-egf (C, D). Full-length na-C11 cannot rescue migration when expressed with MO11, and cannot be rescued by wildtype EC1-3 in the MO11 background (C, D). n=number of embryos scored from at least three independent experiments. Error bars are standard deviation to the mean and a Student's t-test was performed to determine statistical significance. ** < 0.01, *** < 0.005. Scale bar, 500 μm.