Figure 5. Ddb1 deletion induces DNA damage and apoptosis in progenitor cells.
(a) TUNEL staining on tibia sections after polyI:C injection. (b) Percentage of AnnexinV+ cells gated on different populations after polyI:C injection. Western blot (c) and qPCR (d) in lineage negative bone marrows after polyI:C injection. (e) Histogram of intracellular γH2Ax staining gated on HSPC sub-populations and lineage positive cells after polyI:C injection. (f) Immunofluoresence staining of γH2Ax and 53BP1 on flow-sorted LSK cells after polyI:C injection. (g) Representative FACS plots of bone marrow cells 5 days post polyI:C injection. (h) Frequency and absolute number of progenitor cells (n = 4 per group). One of 2 independent experiments was shown. *p<0.05. **p<0.01. ***p<0.001. n.s. p>0.05. (i) Survival curves of control, Trp53-/-::Ddb1f/f::MxCre+ mice, and Ddb1f/f::MxCre+ mice (n = 5 per group, one independent experiment). Age matched littermates on a mixed 129xC57BL/6 background were used for g-i.