Figure 6. Ddb1 deletion is dispensable for mature T cells.
(a) Western blot of DDB1 expression in total thymocytes. (b) Relative Ddb1 mRNA expression in thymocytes. (c) Total cell number of thymus of 6-week old mice (n = 4). (d) Representative FACS plots of thymus and spleen. (e) Spleen CD4+ cells were sorted and stimulated with 1 μg/ml anti-CD3/CD28 in vitro. BrdU incorporation and CFSE dilution was analyzed. (Anti-CD3/CD28 treated CD4+ cells were stained for AnnexinV+ by FACS and percentage of AnnexinV+ cells was shown. (f) Anti-CD3/CD28 treated CD4+ cells were stained for AnnexinV+ by FACS and percentage of AnnexinV+ cells was shown. (g) Anti-CD3/CD28 treated CD4+ cells were labeled and stained with EdU. (h) Percentage of EdU+ cells was shown. More than 300 cells were included in each analysis. (i) Spleen CD4+ cells were treated with 10 μM MG132 for 2 hr and Western blot was performed. Relative Cdkn1acip1 protein levels normalized to β-actin were indicated below the corresponding lanes. (j) qPCR was performed on spleen CD4+ cell. Representative results of two independent experiments were shown. *p<0.05. **p<0.01. ***p<0.001. n.s. p>0.05.