Figure 7. Interaction of Cdkn1acip1 but not p27kip1 with the Cul4-DDB1 complex.
(a) 293T cells were transiently co-transfected with plasmids expressing Myc-tagged Cul4a and Cdkn1acip1. Forty-eight hours post transfection, cells were harvested and lysed. Protein lysates were immunoprecipitated with anti-Myc antibody, and Western blotted for Cdkn1acip1 and DDB1. (b) Similarly as described in (a), p27kip1 was tested for co-immunoprecipitation with the Cul4a-DDB1 complex. (c) Representative FACS analysis of bone marrows after polyI:C injection. Age matched littermates on a mixed 129xC57BL/6 background were used from 2 independent experiments (n = 3–4 per group).