Figure 8. ESC loss of self-renewal after silencing of Ddb1.
(a) Bright field picture of ESC colony 4 day post retroviral transfection. (b) Relative expression of pluripotency genes Oct4, and Nanog and genes representing endoderm (Sox17), mesoderm (Meox1) and ectoderm (GFAP) by qRT-PCR 4 days post selection. (c) Western blot of ESC 4 day post infection or differentiated in the presence of retinoic acid for up to 4 days. (d) Representative FACS blot of Annexin V positive cells. (n=3) n.s.: p>0.05. (e) Representative of cell cycle analysis. (n=3) n.s.: p>0.05.