Figure 1. Absorption spectra of PtAu1afull and dark state recovery kinetics of the LOV domain-containing PtAu1a variants.
(a) Schematic representation of the PtAu1a constructs used in this study. (b) Absorption spectrum of PtAu1afull in the dark (black) and after illumination with blue light (blue). In the dark, the typical signature of an oxidized FMN chromophore can be detected. Upon light activation, these maxima decrease and a new absorption band appears at 390 nm, indicating FMN-cysteine adduct formation. (c) Recovery kinetics of the absorbance at 445 nm of PtAu1afull, PtAu1abZIP-LOV and PtAu1aLOV after light-activation. The red (PtAu1afull), cyan (PtAu1abZIP-LOV) and orange (PtAu1aLOV) lines in the plot represent an exponential fit to the data (black squares). Measurements were performed at a protein concentration of 20 µM and time constants represent the mean of three independent measurements. LOV, light-oxygen-voltage; FMN, flavin mononucleotide.