Table 1.
Purification of the NAD degrading enzymes from P. brevicompactum NRC 829
| Purification steps | Total activity (units) | Protein (mg) | Sp. activity | Recovery (%) | Purification fold |
|---|---|---|---|---|---|
| Alkaline phosphatase | |||||
| Crude extracts | 600 | 390 | 1.5 | 100 | 1 |
| Acetone fraction | 562 | 150 | 3.7 | 93 | 2.5 |
| DEAE-Sephadex A25 | |||||
| ALK1 | 230 | 3.0 | 60.6 | 38 | 51.1 |
| ALK2 | 200 | 2.8 | 71.4 | 33 | 47.6 |
| NAD aminohydrolase | |||||
| Crude extracts | 450 | 390 | 1.1 | 100 | 1 |
| Acetone fraction | 400 | 150 | 2.6 | 88 | 2.2 |
| DEAE-Sephadex A25 | 150 | 5 | 30 | 30 | 27.3 |
| Sephadex G-100 | 100 | 2 | 50 | 22 | 41 |
| NAD glycohydrolase | |||||
| Crude extracts | 290 | 390 | 0.75 | 100 | 1 |
| Acetone-fraction | 245 | 150 | 1.6 | 84 | 2.1 |
| DEAE-Sephadex A 25 | 130 | 5 | 27 | 44 | 36 |
| Sephadex G-100 | 76 | 1.9 | 40 | 26 | 50 |