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. 2016 Jan 21;6:8. doi: 10.1186/s13568-016-0179-y

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© Nishioka et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Display of HAs on yeast cell surfaces. a Scheme of yeast cell surface display of hHAs^α2,6 and hHAs^α2,6*. PI-PLC indicates phosphatidylinositol-specific phospholipase C. hHAs^α2,6* (hSHA^α2,6* and hLHA^α2,6*) represent the hHAs^α2,6 with two point mutations (N190E and D225G). b Immunofluorescence staining of the engineered yeast cells. Scale bars: 5 μm. c Comparison of the display efficiencies as relative fluorescence intensities following the immunofluorescence staining. Values represent mean ± standard error of the mean (SEM) based on three independent experiments