Compound | AsA | Total AsA | ||
---|---|---|---|---|
Test (ml) | Blank (ml) | Test (ml) | Blank (ml) | |
0.2 M K-buffer pH 7.4 | 0.25 | 0.25 | 0.25 | 0.25 |
10 mM DTT | – | – | 0.05 | 0.05 |
Incubation: 10 min at 25 °C | ||||
0.5 % (w/v) NEM | – | – | 0.05 | 0.05 |
ddH2O | 0.1 | 0.1 | – | – |
10 % (w/v) TCA | 0.2 | 0.2 | 0.2 | 0.2 |
44 % (v/v) H3PO4 | 0.2 | 0.2 | 0.2 | 0.2 |
4 % (w/v) 2,2′-dipyridyl | 0.2 | 0.2 | 0.2 | 0.2 |
3 % (w/v) FeCl3 | 0.2 | 0.2 | 0.2 | 0.2 |
Incubation: 40 min at 42 °C |