FIGURE 7.

Physiological changes inN22oe plants. (A) Proline contents in leaves of the N22oe and WT plants grown under normal and drought stress condition. (B) Soluble sugar contents in leaves of the N22oe and WT plants grown under normal and drought stress condition. Four-week-old plants were subjected for drought stress treatment by stopping watering and leaf samples were collected at 6 days after drought treatment. (C) Rates of water loss in detached leaves of the N22oe and WT plants. Leaves were detached from 4-week-old N22oe and WT plants grown under normal conditions and placed on lab bench for drought treatment. Leaf samples were collected at indicated time points and subjected for measuring water loss. (D) Transpiration rate of the N22oe and WT plants. Transpiration rates in six flag leaves of 3-month-old N22oe and WT plants grown under greenhouse condition were determined by Li-6400 instrument at indicated time points. (E) Stomatal aperture of the N22oe and WT plants grown under normal and drought condition. Scale bar = 50 μM. (F) Percentage of open stomata in leaves of the N22oe and WT plants grown under normal and drought condition. Data presented in (A–D,F) are the mean ± SD from three independent experiments and different letters above the columns indicate significant differences at p ≤ 0.05 level with corresponding WT.