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. 2016 Jan 22;7:4. doi: 10.3389/fpls.2016.00004

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Copyright © 2016 Hong, Zhang, Huang, Li and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased ABA content and upregulated expression of ABA biosynthetic and stress-responsive genes in N22oe plants. (A) Increased ABA contents in the N22oe plants. (B) Expression of ABA biosynthesis-related genes in the N22oe plants. (C) Expression of stress-responsive genes in the N22oe plants. Leaf samples were collected from 30-day-old seedlings grown under normal condition and subjected for analyses of ABA content and gene expression. ABA in leaf samples was extracted and quantified by HPLC. FW, fresh weight. Expression of the ABA biosynthetic and stress-responsive genes was analyzed by qRT-PCR and relative expression levels were shown as folds of the level of the actin gene. Data presented are the mean ± SD from three independent experiments and different letters above the columns indicate significant differences at p ≤ 0.05 level with corresponding WT.