Table 3.

Primers selected for use on the prairie cordgrass F₁ progeny.

	pcg_00011	pcg_00012	pcg_00024	pcg_00050*	pcg_00058*	pcg_00059*	pcg_00106*	pcg_1186*	pcg_14142*	pcg_00061	pcg_00062	pcg_7965
PC17_109 (Parent)	GA	GA	TA	TT	GG	CC	CC	CC	GG	TG	GC	AG
PC20_102 (Parent)	GG	GG	AA	CC	TT	GG	GG	TT	CC	TG	GC	AG
13_F1001†	GG	GG	AA	CC	TT	GG	GG	TT	CC	TT	CC	AG
13_F1002	GA	GA	TA	TC	GT	CG	CG	CT	GC	GG	GG	GG
13_F1003	GA	GA	TA	TC	GT	CG	CG	CT	GC	GG	GG	AG
13_F1004	GA	GA	TA	TC	GT	CG	CG	CT	GC	TG	GC	AA
13_F1005	GA	GA	TA	TC	GT	CG	CG	CT	GC	TT	CC	AG
13_F1006	GA	GA	TA	TC	GT	CG	CG	CT	GC	TG	GC	AG
13_F1007†	GG	GG	AA	CC	TT	GG	GG	TT	CC	GG	GG	AG
13_F1008	GG	GG	AA	TC	GT	CG	CG	CT	GC	TG	GC	AA

The first three primers indicate one parent as heterozygous and one parent as homozygous, the next six primers indicate both parents as homozygous for opposite alleles, and the last three primers indicate both parents as heterozygous. Also shown are SNP assay results for eight out of the 83 F₁ hybrids. Indicated are samples that can be identified as true crosses and selfs.

^*Primers that can distinguish true crosses from selfed samples.

^†F₁ individuals that are identified as selfs of the PC20_102 parent.