Fig. 6. CA promoted APAP-induced Nrf2 translocation into nuclear through blocking the interaction between Nrf2 and Keap1. (A) Total or nuclei Nrf2 protein levels were determined by western blot. β-actin and Lamin B1 were used as the endogenous control. The bands were analyzed by densitometry. Data are shown as means±S.E.M. ^*p<0.05 versus APAP alone group. (B) The interaction between Nrf2 and Keap1 in the liver was measured by co-immunoprecipitation assay. Representative data are shown.