Fig. 4. Effects of 1-EBIO on the membrane conductance of HNSCCs with clamped [Ca²⁺]_i.

(A~C) Representative current traces in response to ramp-like pulses (from -100 to 60 mV, holding voltage, -60 mV) and to the application of 50 µM 1-EBIO in HNSCCs. The Ca²⁺ activity of KCl pipette solution was equilibrated to 300 nM with 5 mM EGTA. (D) Summary of I/V curves of the 1-EBIO-induced currents in each cell line (mean±SEM, n=7, respectively). (E) Identification of SK mRNA in HNSCC cancer cells. mRNA was detected for SK1 and SK4 by RT-PCR.