Table 3. Comparison of ECS and glucose uptake-related mRNA in mouse epididymal fat pad after 62 and 118 days of feeding AIN 93M semipurified diets.
| Measurements |
Day 62 |
Day 118 |
Mean testing P-value |
|||||
|---|---|---|---|---|---|---|---|---|
| Control | DHA | Control | DHA | Day 62 | Day 118 | 62 vs118 days control | 62 vs 118 days DHA | |
| CB1 | 1.0±0.1 | 0.4±0.03 | 1.0±0.1 | 0.2±0.01 | ‡ | ‡ | 0.9 | ‡ |
| CB2 | 1.0±0.1 | 1.8±0.2 | 1.0±0.1 | 1.9±0.2 | ‡ | ‡ | 0.9 | 0.5 |
| NAPE-PLD | 1.0±0.1 | 2.1±0.2 | 1.0±0.1 | 2.4±0.2 | ‡ | ‡ | 1 | † |
| FAAH | 1.0±0.1 | 1.0±0.1 | 1.0±0.1 | 1.2±0.1 | 0.8 | † | ‡ | |
| DAGL-α | 1.0±0.1 | 0.6±0.03 | 1.0±0.1 | 0.3±0.02 | ‡ | ‡ | 0.9 | ‡ |
| DAGL-β | 1.0±0.05 | 0.7±0.1 | 1.0±0.04 | 0.4±0.02 | ‡ | ‡ | 1 | ‡ |
| Akt-1 | 1.0±0.1 | 0.7±0.04 | 1.0±0.1 | 0.4±0.01 | ‡ | ‡ | 1 | ‡ |
| Insulin R | 1.0±0.1 | 0.5±0.03 | 1.0±0.1 | 0.5±0.04 | ‡ | ‡ | 1 | † |
| IRS-1 | 1.0±0.1 | 1.5±0.1 | 1.0±0.1 | 1.8±0.1 | ‡ | ‡ | 0.9 | ‡ |
| GLUT4 | 1.0±0.1 | 0.6±0.04 | 1.0±0.1 | 0.3±0.02 | ‡ | ‡ | 1 | ‡ |
| GLUT1 | 1.0±0.1 | 1.6±0.1 | 1.0±0.1 | 2.2±0.1 | ‡ | ‡ | 1 | ‡ |
| Adiponectin | 1.0±0.1 | 1.9±0.1 | 1.0±0.04 | 2.8±0.2 | ‡ | ‡ | 0.9 | ‡ |
| IL-6 | 1.0±0.1 | 0.7±0.04 | 1.0±0.1 | 0.5±0.02 | ‡ | ‡ | 1 | ‡ |
| TNF-α | 1.0±0.05 | 0.5±0.04 | 1.0±0.1 | 0.5±0.03 | ‡ | ‡ | 1 | ‡ |
| MCP1 | 1.0±0.1 | 0.7±0.05 | 1.0±0.1 | 0.6±0.03 | ‡ | ‡ | 1 | ‡ |
| AMPK α2 | 1.0±0.1 | 1.6±0.1 | 1.0±0.1 | 1.6±0.1 | ‡ | ‡ | 1 | 0.4 |
| Adenylate Cyclase | 1.0±0.1 | 1.1±0.04 | 1.0±0.04 | 2.1±0.1 | ‡ | ‡ | 1 | ‡ |
| p42/p44 (MAPK) | 1.0±0.1 | 0.8±0.1 | 1.0±0.1 | 0.6±0.1 | ‡ | ‡ | 1 | † |
| p38 (MAPK) | 1.0±0.1 | 0.5±0.03 | 1.0±0.1 | 0.5±0.03 | ‡ | ‡ | 0.9 | 0.3 |
| JNK (MAPK) | 1.0±0.1 | 0.6±0.1 | 1.0±0.1 | 0.4±0.03 | ‡ | ‡ | 1 | ‡ |
Abbreviations: Akt1, RAC-α serine/threonine-protein kinase (protein kinase B); CB1, cannabinoid receptor 1; CB2, cannabinoid receptor 2; DAGLα, diacylglycerol lipase-α DAGLβ, diacylglycerol lipase-β DHA, docosahexaenoic acid; EAC, endocannabinoid system; FAAH, fatty acid amide hydrolase; GLUT4, glucose transporter type 4; IL-6, interleukin-6; INS-R, insulin receptor; JNK, Jun N-terminal kinase; MAPK, mitogen-activated protein kinase; MyoD1, myogenic differentiation; NAPE-PLD, N-acyl phosphatidylethanolamine phospholipase D; TNF-α, tumor necrosis factor-α.
Values are the mean±s.d. (n=9) of ΔΔCT calculated from mouse tissue mRNA. Differences between diet groups and between time within the same diet groups were compared by t-test.
Mean values of quantitative real-time PCR (qPCR) data were compared by t-test using SAS software.
Significant differences in means: *P<0.05, †P<0.01 and ‡P<0.001.