TABLE 1.
Primers used for qRT-PCR determinations
GenBankTM accession numbers identify transcript recognized by primer pairs.
| Gene | Strand | Sequence 5′–3′ | GenBankTM accession no. |
|---|---|---|---|
| Acp5 | Forward | GACAAGAGGTTCCAGGAGAC | NM_001102404; NM_001102405; NM_007388 |
| Reverse | TTCCAGCCAGCACATACC | ||
| Alpl | Forward | TGGTATGGGCGTCTCCACAGTAACC | NM_007431; NM_001287172; NM_001287176 |
| Reverse | CTTGGAGAGGGCCACAAAGG | ||
| Bglap | Forward | GACTCCGGCGCTACCTTGGGTAAG | NM_001037939 |
| Reverse | CCCAGCACAACTCCTCCCTA | ||
| Casp3 | Forward | ATGGAGAACAACAAAACCTC | NM_009810; NM_001284409 |
| Reverse | CCATGTATGGTCTTTACTTCA | ||
| Gapdh | Forward | CCCCTCTGGAAAGCTGTGGCGT | NM_008084; NM_001289726 |
| Reverse | AGCTTCCCGTTCAGCTCTGG | ||
| Hes1 | Forward | ACCAAAGACGGCCTCTGAGCACAGAAAGT | NM_008235 |
| Reverse | ATTCTTGCCCTTCGCCTCTT | ||
| Hey1 | Forward | ATCTCAACAACTACGCATCCCAGC | NM_010423 |
| Reverse | GTGTGGGTGATGTCCGAAGG | ||
| Hey2 | Forward | AGCGAGAACAATTACCCTGGGCAC | NM_013904 |
| Reverse | GGTAGTTGTCGGTGAATTGGACCT | ||
| HeyL | Forward | CAGTAGCCTTTCTGAATTGCGAC | NM_013905 |
| Reverse | AGCTTGGAGGAGCCCTGTTTC | ||
| Nfatc1 | Forward | GCGCAAGTACAGTCTCAATGGCC | NM_198429; NM_001164110; NM_001164111; NM_001164112; NM_00116641091; NM_016791 |
| Reverse | GGATGGTGTGGGTGAGTGGT | ||
| Notch2 | Forward | CATCGTGACTTTCCA | NM_010928 |
| Reverse | GGATCTGGTACATAGAG | ||
| Rpl38 | Forward | AGAACAAGGATAATGTGAAGTTCAAGGTTC | NM_001048057; NM_001048058; NM_023372 |
| Reverse | CTGCTTCAGCTTCTCTGCCTTT | ||
| Tnfrsf11b | Forward | CAGAAAGGAAATGCAACACATGACAAC | NM_008764 |
| Reverse | GCCTCTTCACACAGGGTGACATC | ||
| Tnfsf11 | Forward | TATAGAATCCTGAGACTCCATGAAAAC | NM_011613 |
| Reverse | CCCTGAAAGGCTTGTTTCATCC |