FIGURE 2.

Critical role of a new distal AP-1-binding site in TF promoter. A, schematic description of human TF gene promoter and relevant transcription factor-binding sites. The new distal AP-1-binding site at −1363 bp is well conserved across multiple species (B). HCAEC were transfected with different luciferase plasmid constructs (C) and stimulated with UTP (100 μm) for 4 h, after which levels of luciferase activity relative to non-stimulated control were corrected for transfection efficiency, and summarized data are shown on the right. Top bar in white indicates result from co-treatment with UTP and actinomycin D (10 μg/ml). (n = 5, *, p < 0.05).