FIGURE 3.

Evidence of nuclear protein binding to the new distal AP-1 site and its regulation by P2Y2R. A, HCAEC nuclear protein was isolated after treatment of the cells with UTP (lanes 4–6) or TNF-α (lanes 7–9) or vehicle (lanes 1–3) for 30 min, incubated with unlabeled and/or biotin-labeled probes, fractionated by electrophoresis, and visualized by ECL detection after the membrane was incubated with horseradish peroxidase-conjugated streptavidin. B, summarized data for band density of A were quantified with Quantity One. n = 4, *, p < 0.05.