FIGURE 7.

Differential roles of AP-1 subunits in P2Y2R-mediated TF gene transcription. A--C, c-Jun, Fra-1, and ATF-2 protein levels were determined by Western blotting after HCAEC were transfected with their respective siRNA for 48 h. UTP-induced TF pre-mRNA was evaluated after c-Jun, Fra-1, or ATF-2 was knocked down by siRNA in HCAEC. *, p < 0.05 versus UTP-stimulated cells were transfected with scrambled siRNA (D). n = 5. UTP-induced TF protein expression under the same siRNA intervention was determined by Western blotting (E). Data were representative of three independent experiments. ctr, control.