FIGURE 11.
Analysis of mitochondrial bioenergetics in Tspofl/fl and Tspo−/− fibroblasts. A, baseline OCR and maximal OCR after addition of the protonophore FCCP was significantly lower in Tspo−/− compared with Tspofl/fl fibroblasts. OCR declined upon blocking ATP synthase activity using oligomycin or inhibiting electron transport chain using antimycin A and rotenone, but they were not significantly different between the two genotypes. B, mitochondrial coupling efficiency was not different between Tspofl/fl and Tspo−/− fibroblasts. C, mitochondrial ATP production was significantly lower in Tspo−/− compared with Tspofl/fl fibroblasts. D, proton leak was significantly lower in Tspo−/− compared with Tspofl/fl fibroblasts. E, spare respiratory capacity was significantly lower in Tspo−/− compared with Tspofl/fl fibroblasts. *, p < 0.05 (n = 5–7 independent primary cultures).