FIGURE 5.
PTH enhances osteogenic differentiation of Gsα-deficient osteoblasts. A, Alizarin red staining of Gsα(fl/fl) calvarial osteoblasts infected with adeno-β-gal (control) or adeno-Cre, then treated with PBS or PTH for 6 h of every 48 h, and subjected to osteogenic differentiation for 7 and 14 days. B, quantitation of mineralized nodule area at day 14. C, numbers of cells per well of Gsα(fl/fl) calvarial osteoblasts treated with adeno-β-gal or adeno-Cre and then with PBS or PTH. n = 3 experiments. D, mRNA expression levels of Runx2, Osx, ColIα1, Opn, and Bglap at day 7 in cells described above. Mean results from three replicate experiments are shown. *, p < 0.05 versus respective PBS control; #, p < 0.05 versus respective WT control. Error bars represent S.E.