TABLE 1.
Histomorphometry of 12-week-old control (WT) or P-GsαOsxKO (KO) tibiae with intermittent PTH or vehicle for 4 weeks
Values are mean ± S.E. with n = 6–9 mice for each group. BFR, bone formation rate; BS, bone surface; MS, mineralizing surface; MAR, mineral apposition rate; Ob.S, osteoblast surface; N.Ob/B.Pm, number of osteoblasts per bone perimeter; O.Th, osteoid thickness; OS, osteoid surface; Os.S, osteoclast surface; N.Oc/B.Pm, number of osteoclasts per bone perimeter.
| Parameters | WT |
KO |
||
|---|---|---|---|---|
| PBS (n = 8) | PTH (n = 9) | PBS (n = 6) | PTH (n = 6) | |
| BV/TV (%) | 15.50 ± 1.93 | 18.96 ± 2.27 | 5.22 ± 0.83a | 5.48 ± 1.47a |
| Tb.Th (μm) | 37.84 ± 1.79 | 48.98 ± 2.38b | 28.62 ± 1.29 | 37.29 ± 3.24a,b |
| Tb.N (/mm) | 4.00 ± 0.35 | 3.78 ± 0.31 | 1.79 ± 0.23a | 1.56 ± 0.41a |
| Tb.Sp (μm) | 229.57 ± 30.75 | 232.41 ± 27.59 | 576.35 ± 75.42 | 1324.96 ± 622.91 |
| MS/BS (%) | 22.50 ± 4.22 | 30.61 ± 2.22 | 27.68 ± 1.60 | 37.30 ± 5.59a |
| MAR (μm/day) | 1.57 ± 0.16 | 3.00 ± 0.26b | 1.21 ± 0.34 | 1.86 ± 0.07a |
| BFR/BS (μm3/μm2/day) | 0.35 ± 0.08 | 0.93 ± 0.12b | 0.33 ± 0.09 | 0.70 ± 0.11b |
| Ob.S/BS (%) | 6.53 ± 1.30 | 12.37 ± 1.67b | 1.21 ± 0.77 | 8.22 ± 2.27b |
| N.Ob/B.Pm (/mm) | 4.08 ± 0.89 | 8.19 ± 1.35b | 0.76 ± 0.49 | 5.35 ± 1.56b |
| OS/BS (%) | 2.05 ± 0.64 | 6.49 ± 1.91 | 0.16 ± 0.16 | 2.91 ± 1.05b |
| O.Th (μm) | 0.60 ± 0.11 | 0.87 ± 0.22 | 0.05 ± 0.05 | 0.48 ± 0.16 |
| Oc.S/BS (%) | 4.97 ± 0.71 | 5.31 ± 0.32 | 5.03 ± 0.73 | 6.67 ± 0.60 |
| N.Oc/B.Pm (/mm) | 2.27 ± 0.33 | 2.17 ± 0.13 | 2.86 ± 0.47 | 3.34 ± 0.35 |
a p < 0.05 versus respective WT control.
b p < 0.05 versus respective PBS control.