. 2015 Oct 30;291(4):1751–1762. doi: 10.1074/jbc.M115.686121

TABLE 3.

List of primers used for construction of expression plasmids

Name	Figure	PCR template	Nucleotide sequence (5′ to 3′)
pIZT_BmKr-h1	Fig. 2B, 3A, and 6A	Full length cDNA clone	`AAAAAGCAGGCTTCGAAGGAGATAGAACCATGATAGGTGACGAGGAGCGAG`
pIZT_BmKr-h1	Fig. 2B, 3A, and 6A	Full length cDNA clone	`AGAAAGCTGGGTCCTATGATTCTGTAGCTGGCGGAG`
pIZT_p65AD_BmKr-h1	Figs. 3, A, B, C, D, F, and G; 5, A and B	pIZT_BmKr-h1	`ATGATAGGTGACGAGGAGCGAGTTCATC`
			`GGTTCTATCTCCTTCGAAGCCTGCTTTTTT`
			`ATGTTCCCTTCTGGGCAAATCTCAAACCAGG`
			`TTGGCCGCTGGAGCTGATCTGACTC`
pIZT_p65AD		pIZT_p65AD_BmKr-h1	`ACCCAGCTTTCTTGTACAAAGTGGTCCC`
pIZT_p65AD		pIZT_p65AD_BmKr-h1	`CTATTGGCCGCTGGAGCTGATCTGACTC`
pF25A_BmKr-h1		pIZT_BmKr-h1	`TTTGCGATCGCATGATAGGTGACGAGGAGCGAG`
pF25A_BmKr-h1		pIZT_BmKr-h1	`TTTGTTTAAACCTATGATTCTGTAGCTGGCGGAGCAAATTG`
pF25A_BmKr-h1_HA	Fig. 4, A and B	pF25A_BmKr-h1	`TCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTTGAGTTTAAACGAATTCGGGCTCGGTACCC`
			`CGTCATAGGGATAGCCCGCATAGTCAGGAACATCGTATGGGTATGATTCTGTAGCTGGCGGAGCAAATTGTATAAC`
pF25A_BmKr-h1(1–243)	Fig. 4B	pF25A_BmKr-h1	`TGAGTTTAAACGAATTCGGGCTCGGTACCC`
pF25A_BmKr-h1(1–243)	Fig. 4B	pF25A_BmKr-h1	`ACTCTGTAATGGTGCGGTGCGGGG`
pIZT_p65AD_BmKr-h1_ΔZF1	Fig. 5A	pIZT_p65AD_BmKr-h1	`CGTTCTACTGCTGATGCACATCGCTGC`
pIZT_p65AD_BmKr-h1_ΔZF1	Fig. 5A	pIZT_p65AD_BmKr-h1	`CTGATGAACTCGCTCCTCGTCACCTATC`
pIZT_p65AD_BmKr-h1_ΔZF2	Fig. 5A	pIZT_p65AD_BmKr-h1	`ACTGGCGAGCGACCATTTGAATGTGAATATTG`
pIZT_p65AD_BmKr-h1_ΔZF2	Fig. 5A	pIZT_p65AD_BmKr-h1	`GCGATGTGCATCAGCAGTAGAACGATG`
pIZT_p65AD_BmKr-h1_ΔZF3	Fig. 5A	pIZT_p65AD_BmKr-h1	`ACAAAGGAGAGGCCGTATCGGTGCAATG`
pIZT_p65AD_BmKr-h1_ΔZF3	Fig. 5A	pIZT_p65AD_BmKr-h1	`TTCAAATGGTCGCTCGCCAGTATGGGTTC`
pIZT_p65AD_BmKr-h1_ΔZF4	Fig. 5A	pIZT_p65AD_BmKr-h1	`ACCGGCGAGAGACCGCACGCATG`
pIZT_p65AD_BmKr-h1_ΔZF4	Fig. 5A	pIZT_p65AD_BmKr-h1	`CCGATACGGCCTCTCCTTTGTGTGAATAC`
pIZT_p65AD_BmKr-h1_ΔZF5	Fig. 5A	pIZT_p65AD_BmKr-h1	`ACAGGTGAGAAACCCTATCGTTGTCCTG`
pIZT_p65AD_BmKr-h1_ΔZF5	Fig. 5A	pIZT_p65AD_BmKr-h1	`TGCGTGCGGTCTCTCGCCGGTAT`
pIZT_p65AD_BmKr-h1_ΔZF6	Fig. 5A	pIZT_p65AD_BmKr-h1	`ACGGGAGAGCGACCCTATACTTGTG`
pIZT_p65AD_BmKr-h1_ΔZF6	Fig. 5A	pIZT_p65AD_BmKr-h1	`ACGATAGGGTTTCTCACCTGTGTGGG`
pIZT_p65AD_BmKr-h1_ΔZF7	Fig. 5A	pIZT_p65AD_BmKr-h1	`TTTGGCGAGCGCTGTTATCGTTGCACTG`
pIZT_p65AD_BmKr-h1_ΔZF7	Fig. 5A	pIZT_p65AD_BmKr-h1	`AGTATAGGGTCGCTCTCCCGTGTGAG`
pIZT_p65AD_BmKr-h1_ΔZF8	Fig. 5A	pIZT_p65AD_BmKr-h1	`GGCGCGGAAGCCCCCCGCA`
pIZT_p65AD_BmKr-h1_ΔZF8	Fig. 5A	pIZT_p65AD_BmKr-h1	`GCGCTCGCCAAAATGTTGAAAACGATGTAAT`
pIZT_p65AD_BmKr-h1_ZF1_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`GCAGTGCGCTTACAGCATTCGCACGATCCTTTCGTTC`
pIZT_p65AD_BmKr-h1_ZF1_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`GGGTGGACAATGTCAAACCAGACTCGCCGGACTGAT`
pIZT_p65AD_BmKr-h1_ZF2_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`CTGCACGACTCGTGCGCTTCTACCGAACCTTTACTGG`
pIZT_p65AD_BmKr-h1_ZF2_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`GCACAGCAAAAGTCTTATGGGACACGTCGGAGCGAT`
pIZT_p65AD_BmKr-h1_ZF3_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`AAGAAAATTTGCAAGTATTCCGTCGTATTTTCACAAA`
pIZT_p65AD_BmKr-h1_ZF3_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`TCACACTAAACATTTTATGGGAATATTCAGATTCAA`
pIZT_p65AD_BmKr-h1_ZF4_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`CCGGAAAACTTCACAGATTTGCTAGAATCTTTACCGG`
pIZT_p65AD_BmKr-h1_ZF4_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`AATGCTCGAATGCCGCATCAGAAACATTGGACCGAT`
pIZT_p65AD_BmKr-h1_ZF5_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`CTGGTCAACTGGTCATATTTTTAAGGACCTTCACAGG`
pIZT_p65AD_BmKr-h1_ZF5_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`ATTGTATGAACGTCTTATGAGAATGTGGAGATGCGT`
pIZT_p65AD_BmKr-h1_ZF6_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`TCCTCTAAACAGCTTAAAGTATTTTCTCGTACTTTCACGGG`
pIZT_p65AD_BmKr-h1_ZF6_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`GGTGAAGCCCTTTCCGGATCCAGGTGCAGGAGAACGAT`
pIZT_p65AD_BmKr-h1_ZF7_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`ATCATGTTCTCAAATTATTTCGTTTTCAATTTTTTGG`
pIZT_p65AD_BmKr-h1_ZF7_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`TATAACCAAAGTCCCTGAGAGAAATTTCAGAAGTAT`
pIZT_p65AD_BmKr-h1_ZF8_amino acid substitution	Fig. 5B	pIZT_p65AD_BmKr-h1	`CAAAAAAACAAATGGAAGCCTTTATTTACAAAGAATTTGGCGC`