TABLE 4.
List of primers and oligonucleotides used for dsRNA syntheses and EMSA
q-PCR, quantitative real-time PCR.
| Name | Figure | PCR template | Nucleotide sequence (5′ to 3′) |
|---|---|---|---|
| dsRNA | |||
| dsMalE | Fig. 2, C and D | pMAL-c4E (NEB) | GGATCCTAATACGACTCACTATAGGTGATTGCTGCTGACGGGGGT |
| GGATCCTAATACGACTCACTATAGGTTTCTGGGCGTTTTCCATAGTGG | |||
| dsBmKr-h1 | Fig. 2, C and D | pIZT_BmKr-h1 | GGATCCTAATACGACTCACTATAGGATGATAGGTGACGAGGAGCGAG |
| GGATCCTAATACGACTCACTATAGGGCTCTCCCGTGTGAGTACGAG | |||
| qPCR | |||
| BmKr-h1_RNAi_qPCR | Fig. 2C | cDNA | CATCGTTTTCAACATTTTGGCGAG |
| CACATCACTTTACCATCGGCAGC | |||
| EMSA | |||
| Probe (−4480/−4401) | Fig. 4, A and B | Biotin-ON-tgtgcgctctACGTCTATCCGTGACCTACGCTAACGCTAAATAGAGTTCCGACCTTGACTCTAACGGTCGtcgactctac | |
| Biotin-ON-gtagagtcgaCGACCGTTAGAGTCAAGGTCGGAACTCTATTTAGCGTTAGCGTAGGTCACGGATAGACGTagagcgcaca | |||
| Specific competitor (−4480/−4401) | Fig. 4, A and B | tgtgcgctctACGTCTATCCGTGACCTACGCTAACGCTAAATAGAGTTCCGACCTTGACTCTAACGGTCGtcgactctac | |
| gtagagtcgaCGACCGTTAGAGTCAAGGTCGGAACTCTATTTAGCGTTAGCGTAGGTCACGGATAGACGTagagcgcaca | |||
| Non-specific competitor (−4054/−3975) | Fig. 4, A and B | TTAAGAAGTTTCACTTCCGATTTGTTTTATTAAACTAATCAAGTTGTTTTCATTCTGAAATATTATTATTTGAGAGACTA | |
| TAGTCTCTCAAATAATAATATTTCAGAATGAAAACAACTTGATTAGTTTAATAAAACAAATCGGAAGTGAAACTTCTTAA | |||