TABLE 3.
PFC1→C2
Shown is a quantitative description of the change in deuterium uptake rates between different experimental conditions, averaged over peptides measured in each experiment. When PFC1→C2 is less than 1, C1 exchanges more slowly than C2, and when it is greater than 1, C2 exchanges more slowly than C1, as described under the “Experimental Procedures.” In Fig. 5A, the bold entries in this table, indicating peptides that respond differentially to at least one of the experimental variables, are converted to changes in free energy between the indicated conditions (δδGC1→C2). H refers to heavy chain in constructions such as H5–H17, and L refers to light chain in constructions such as L136–L148.
| Peptide | PFYTE-WTa |
PFpH 5.5-7a |
PFpH 5.5 - pH5.5+FcRna |
||||||
|---|---|---|---|---|---|---|---|---|---|
| pH 7 | pH 5.5 | pH 5.5 + FcRn | WT | YTE | YTE/WT | WT | YTE | YTE/WT | |
| H5–H17 | 1 | 1 | 1 | 1.0–2.2 | 1.0–2.2 | 1 | 1 | 1 | 1 |
| H36–H47 | 1 | 1 | 1 | 1.5–1.8 | 1.5–1.8 | 1 | 1 | 1 | 1 |
| H97–H106 | 1 | 1 | 1 | 1–5 | 1–5 | 1 | 1 | 1 | 1 |
| H146–H155 | 1 | 1 | 1 | 1.5–1.8 | 1.5–1.8 | 1 | 1 | 1 | 1 |
| H241–H252 | 11 | 9.3 | 0.7–1.5b | 6.1 | 5.2 | 0.9 | 16 | 120 | 7.5 |
| H253–H262 | 0.4 | 0.5–1.5b | 0.3 | 4.5 | 13 | 2.9 | 31 | 110 | 3.5 |
| H263–H277 | 1 | 1 | 1 | 1.8–2.3 | 1.8–2.3 | 1 | 1 | 1 | 1 |
| H278–H293 | 0.5 | 0.85 | 1 | 1.8–2.2 | 1.8–2.2 | 1 | 1 | 1 | 1 |
| H306–H318 | 1 | 1.5 | 0.4–1.2b | 11 | 13 | 1.2 | 4.5 | 4.5 | 1 |
| H336–H348 | 5 | 2.9 | 1 | < 1.5 | 3.8 | 3.8 | 1 | 2 | 2.0 |
| H369–H379 | 1 | 0.4 | 0.2 | 2.4 | 3 | 1.3 | 1.6 | 3 | 1.9 |
| H379–H390 | 1 | 0.8 | 0.85 | 2.2 | 2.4 | 1.1 | 1 | 1 | 1 |
| H429–H440 | 2.3 | 6.3 | 0.2 | 21 | 24 | 1.1 | 25 | 90 | 3.6 |
| L136–L148 | 1 | 1 | 1 | 1.65 | 1.65 | 1 | 1 | 1 | 1 |
| L162–L172 | 1 | 1 | 1 | 2.1–2.3 | 2.1–2.3 | 1 | 1 | 1 | 1 |
| L196–L206 | 1 | 1 | 1 | 2–3 | 2–3 | 1 | 1 | 1 | 1 |
a PF = 1 if no significant differences were found by t test or if value was less than 1.5 threshold (see “Results”).
b Some sites are faster, others are slower.