FIGURE 2.

Bach2 phosphorylation in Pten-deficient B cells. A, whole cell lysates from B cells from wild type mice were treated or not with alkaline phosphatase (CIAP) for 1 h, and the Bach2 and Pten proteins were detected by immunoblotting (IB) using antibodies against Bach2 or Pten proteins. Pten is shown as a loading control. B, splenic B cells derived from Pten^+/+ mice (left, WT) or Pten^−/− mice (right, KO) at 6 days after the last pIpC administration were incubated or not for 1 h with anti-IgM stimulation. Whole cell lysates were prepared and were treated with CIAP for 1 h where indicated. Immunoblotting was performed with antibodies against the indicated proteins or phosphoproteins. Tubulin is shown as a loading control. The data are representative of three experiments.