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. 2015 Nov 4;291(4):1866–1876. doi: 10.1074/jbc.M115.677484

TABLE 3.

Data collection and scattering-derived parameters for SpPaaI SAXS data

SpPaaI
Data collection parameters
    Instrument Pilatus 1 m
    Beam geometry 250 × 150 μm
    Wavelength (Å) 1.54
    q range (Å−1) 0.01–0.5
    Exposure time (s) 21.0
    Concentration range (mg ml−1) 0.3 - 5
    Temperature (C) 26

Structural parameters
    I (0) (cm−1) (from P (r)) 0.03
    Rg (Å) (from P (r)) 24.83
    I (0) (cm−1) (from Guinier) 0.03
    Rg (Å) (from Guinier) 24.93
    Dmax (Å) 67
    Porod volume estimate (Å3) 89, 294
    Dry volume calculated from sequence (Å3) 18, 413

Molecular mass determination
    Partial specific volume (cm3 g−1) 0.75
    Molecular mass (Da) (from I (0)) 63,000
    Calculated monomeric molecular mass from sequence (Da) 60,000